mageck-crispr-analyzer
MAGeCK CRISPR screen analysis skill for gene essentiality scoring
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技能内容
MAGeCK CRISPR Analyzer Skill
Purpose
Provide MAGeCK CRISPR screen analysis for gene essentiality scoring.
Capabilities
- Guide read counting
- Normalization and QC
- Gene-level analysis
- Pathway enrichment
- MLE model for complex designs
- Visualization outputs
Usage Guidelines
- Count guides accurately from sequencing data
- Normalize counts appropriately
- Calculate gene-level scores
- Enrich for biological pathways
- Use MLE for complex experimental designs
- Generate informative visualizations
Dependencies
- MAGeCK
- BAGEL
- CRISPResso2
Process Integration
- CRISPR Screen Analysis (crispr-screen-analysis)
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原文件路径
library/specializations/domains/science/bioinformatics/skills/mageck-crispr-analyzer/SKILL.md