blast-sequence-search
BLAST skill for sequence similarity searching, homology detection, and database querying
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技能内容
BLAST Sequence Search Skill
Purpose
Provide BLAST capabilities for sequence similarity searching, homology detection, and database querying across nucleotide and protein sequences.
Capabilities
- BLASTn/BLASTp/BLASTx execution
- Custom database creation and management
- E-value and alignment filtering
- Output parsing and result annotation
- Batch query processing
- Remote NCBI database queries
Usage Guidelines
- Select appropriate BLAST program for query/database type
- Set E-value thresholds based on search sensitivity needs
- Create custom databases for project-specific searches
- Parse and filter results for downstream analysis
- Consider computational resources for large searches
- Document database versions for reproducibility
Dependencies
- NCBI BLAST+
- DIAMOND
- MMseqs2
Process Integration
- Whole Genome Sequencing Pipeline (wgs-analysis-pipeline)
- Protein Structure Prediction (protein-structure-prediction)
- Shotgun Metagenomics Pipeline (shotgun-metagenomics)
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原文件路径
library/specializations/domains/science/bioinformatics/skills/blast-sequence-search/SKILL.md